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Alibaba.com offers 1,680 double digestion products. A wide variety of double digestion options are available to you, such as dosage form, function, and certification. Double the volume of the refolding mixture with 100 mM Tris, pH 8.0. The concentration of the Guanidine HCl must be below 250 mM for the ULP1 digestion to be effective. 2.

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Double Digest Finder. Use this tool to guide your reaction buffer selection when setting up double-digests, a common timesaving procedure.

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Setting up a Double Digestion. Double digests with NEB's restriction enzymes can be set up in rCutSmart Buffer™. Otherwise, choose an NEBuffer that results in the most activity for both enzymes. DoubleDigest conveniently calculates the best enzymatic reaction buffer, enzyme concentrations, incubation conditions, and any additives needed in your double digest reaction.

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